Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities.

ABSTRACT: Techniques based on high-throughput sequencing (HTS) of environmental DNA have provided a new way of studying fungal diversity. However, these techniques suffer from a number of methodological biases which may appear at any of the steps involved in a metabarcoding study. Air is one of the most important environments where fungi can be found, because it is the primary medium of dispersal for many species. Looking ahead to future developments, it was decided to test 20 protocols, including different passive spore traps, spore recovery procedures, DNA extraction kits, and barcode loci. HTS was performed with the Illumina MiSeq platform targeting two subloci of the fungal internal transcribed spacer. Multivariate analysis and generalized linear models showed that the type of passive spore trap

SUBMITTER: Aguayo J 

PROVIDER: S-EPMC5960964 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature