Bilayer Thickness and Curvature Influence Binding and Insertion of a pHLIP Peptide.

ABSTRACT: The physical properties of lipid bilayers, such as curvature and fluidity, can affect the interactions of polypeptides with membranes, influencing biological events. Additionally, given the growing interest in peptide-based therapeutics, understanding the influence of membrane properties on membrane-associated peptides has potential utility. pH low insertion peptides (pHLIPs) are a family of water-soluble peptides that can insert across cell membranes in a pH-dependent manner, enabling the use of pH to follow peptide-lipid interactions. Here we study pHLIP interactions with liposomes varying in size and composition, to determine the influence of several key membrane physical properties. We find that pHLIP binding to bilayer surfaces at neutral pH is governed by the ease of access to the me

SUBMITTER: Karabadzhak AG 

PROVIDER: S-EPMC5961746 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature