Single-Molecule Light-Sheet Imaging of Suspended T Cells.

ABSTRACT: Adaptive immune responses are initiated by triggering of the T cell receptor. Single-molecule imaging based on total internal reflection fluorescence microscopy at coverslip/basal cell interfaces is commonly used to study this process. These experiments have suggested, unexpectedly, that the diffusional behavior and organization of signaling proteins and receptors may be constrained before activation. However, it is unclear to what extent the molecular behavior and cell state is affected by the imaging conditions, i.e., by the presence of a supporting surface. In this study, we implemented single-molecule light-sheet microscopy, which enables single receptors to be directly visualized at any plane in a cell to study protein dynamics and organization in live, resting T cells. The light shee

SUBMITTER: Ponjavic A 

PROVIDER: S-EPMC5961759 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature