CircSMAD2 inhibits the epithelial-mesenchymal transition by targeting miR-629 in hepatocellular carcinoma.

ABSTRACT:

Background

Circular RNAs (circRNAs) are a class of widely distributed non-coding RNAs, which drew little attention for decades. Recent studies show that circRNAs are involved in cancer progression.

Methods

The circSMAD2 expression in HCC and adjacent non-tumor tissues was measured by quantitative real-time polymerase chain reaction, and the biological function of circSMAD2 was explored by proliferation, apoptosis, migration, invasion, and Western blot assays. Next, the dual-luciferase reporter assay was performed to identify the target miRNA of circSMAD2. Finally, circSMAD2 and its target miRNA were co-transfected in HCC cells to investigate their relationship to HCC progression.

Results

In this study, we found that circRNA SMAD2 (circSMAD2) expression was downregulat

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5962255 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature