Neuromodulation of orexin neurons reduces diet-induced adiposity.

ABSTRACT:

Background/objectives

Low levels of orexin are associated with obesity and reduced physical activity in humans and animals.

Subjects/methods

Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) selectively activated orexin neurons in mouse lateral hypothalamus (LH) to measure effects on spontaneous physical activity (SPA). DREADD targeting was achieved by stereotaxic injection of AAV vectors into caudal lateral LH of heterozygous orexin-Cre or C57/B6J mice. In one set of studies, excitation of orexin neurons was examined (virus: AAV2-EF1a-DIO-hM3Dq-mCherry), and test sessions began 3-4 h after light cycle onset. In a study examining the inhibition of orexin neurons (virus: AAV2-hSyn-DIO-hM4Di-mCherry), testing began 15 min prior to dark cycle onset. Clozapine

SUBMITTER: Zink AN 

PROVIDER: S-EPMC5962366 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature