Impact of Mitochondrial Permeability on Endothelial Cell Immunogenicity in Transplantation.

ABSTRACT:

Background

Microvascular endothelial cells (ECs) are central to an allograft's immunogenicity. Cold ischemia and reperfusion injury associated with static cold storage and warm reperfusion activates ECs and increases the immunogenicity of the allograft. After reperfusion, mitochondrial permeability transition pore (mPTP) opening contributes to mitochondrial dysfunction in the allograft, which correlates to alloimmune rejection. Current understanding of this relationship, however, centers on the whole allograft instead of ECs. This study aimed to elucidate the relationship between EC mPTP opening and their immunophenotype.

Methods

Mitochondrial metabolic fitness and glycolysis in ECs were assessed in parallel with metabolic gene microarray postreperfusion. NIM811 was used to

SUBMITTER: Tran DT 

PROVIDER: S-EPMC5962417 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature