Quantitative Live-Cell Confocal Imaging of 3D Spheroids in a High-Throughput Format.

ABSTRACT: Accurately predicting the human response to new compounds is critical to a wide variety of industries. Standard screening pipelines (including both in vitro and in vivo models) often lack predictive power. Three-dimensional (3D) culture systems of human cells, a more physiologically relevant platform, could provide a high-throughput, automated means to test the efficacy and/or toxicity of novel substances. However, the challenge of obtaining high-magnification, confocal z stacks of 3D spheroids and understanding their respective quantitative limitations must be overcome first. To address this challenge, we developed a method to form spheroids of reproducible size at precise spatial locations across a 96-well plate. Spheroids of variable radii were labeled with four different fluorescent dy

SUBMITTER: Leary E 

PROVIDER: S-EPMC5962438 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature