Transcription and microRNA Profiling of Cultured Human Tympanic Membrane Epidermal Keratinocytes.

ABSTRACT: The human tympanic membrane (TM) has a thin outer epidermal layer which plays an important role in TM homeostasis and ear health. The specialised cells of the TM epidermis have a different physiology compared to normal skin epidermal keratinocytes, displaying a dynamic and constitutive migration that maintains a clear TM surface and assists in regeneration. Here, we characterise and compare molecular phenotypes in keratinocyte cultures from TM and normal skin. TM keratinocytes were isolated by enzymatic digestion and cultured in vitro. We compared global mRNA and microRNA expression of the cultured cells with that of human epidermal keratinocyte cultures. Genes with either relatively higher or lower expression were analysed further using the biostatistical tools g:Profiler and Ingenuity Pa

SUBMITTER: Aabel P 

PROVIDER: S-EPMC5962475 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature