The major transcription initiation site of the p27Kip1 gene is conserved in human and mouse and produces a long 5'-UTR.

ABSTRACT:

Background

The cyclin-dependent kinase inhibitor p27Kip1 is essential for proper control of cell cycle progression. The levels of p27Kip1 are regulated by several mechanisms including transcriptional and translational controls. In order to delineate the molecular details of these regulatory mechanisms it is important to identify the transcription initiation site within the p27Kip1 gene, thereby defining the promoter region of the gene and the 5'-untranslated region of the p27Kip1 mRNA. Although several previous studies have attempted to map p27Kip1 transcription start sites, the results vary widely for both the mouse and human genes. In addition, even though the mouse and human p27Kip1 gene sequences are very highly conserved, the reported start sites are notably different.

Resu

SUBMITTER: Coleman J 

PROVIDER: S-EPMC59625 | biostudies-literature | 2001

REPOSITORIES: biostudies-literature