Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes.

ABSTRACT: The Cas9 endonuclease can be programmed by guide RNA to introduce sequence-specific breaks in genomic DNA. Thus, Cas9-based approaches present a range of novel options for genome manipulation and precision editing. African trypanosomes are parasites that cause lethal human and animal diseases. They also serve as models for studies on eukaryotic biology, including 'divergent' biology. Genome modification, exploiting the native homologous recombination machinery, has been important for studies on trypanosomes but often requires multiple rounds of transfection using selectable markers that integrate at low efficiency. We report a system for delivering tetracycline inducible Cas9 and guide RNA to Trypanosoma brucei. In these cells, targeted DNA cleavage and gene disruption can be achieved at c

SUBMITTER: Rico E 

PROVIDER: S-EPMC5962531 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature