Transcriptional timing and noise of yeast cell cycle regulators-a single cell and single molecule approach.

ABSTRACT: Gene expression is a stochastic process and its appropriate regulation is critical for cell cycle progression. Cellular stress response necessitates expression reprogramming and cell cycle arrest. While previous studies are mostly based on bulk experiments influenced by synchronization effects or lack temporal distribution, time-resolved methods on single cells are needed to understand eukaryotic cell cycle in context of noisy gene expression and external perturbations. Using smFISH, microscopy and morphological markers, we monitored mRNA abundances over cell cycle phases and calculated transcriptional noise for SIC1, CLN2, and CLB5, the main G1/S transition regulators in budding yeast. We employed mathematical modeling for in silico synchronization and for derivation

SUBMITTER: Amoussouvi A 

PROVIDER: S-EPMC5962571 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature