Imaging glutathione depletion in the rat brain using ascorbate-derived hyperpolarized MR and PET probes.

ABSTRACT: Oxidative stress is a critical feature of several common neurologic disorders. The brain is well adapted to neutralize oxidative injury by maintaining a high steady-state concentration of small-molecule intracellular antioxidants including glutathione in astrocytes and ascorbic acid in neurons. Ascorbate-derived imaging probes for hyperpolarized ¹³C magnetic resonance spectroscopy and positron emission tomography have been used to study redox changes (antioxidant depletion and reactive oxygen species accumulation) in vivo. In this study, we applied these imaging probes to the normal rat brain and a rat model of glutathione depletion. We first studied hyperpolarized [1-¹³C]dehydroascorbate in the normal rat brain, demonstrating its robust conversion to [1-¹³

SUBMITTER: Qin H 

PROVIDER: S-EPMC5962598 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature