The Chaperonin GroEL: A Versatile Tool for Applied Biotechnology Platforms.

ABSTRACT: The nucleotide-free chaperonin GroEL is capable of capturing transient unfolded or partially unfolded states that flicker in and out of existence due to large-scale protein dynamic vibrational modes. In this work, three short vignettes are presented to highlight our continuing advances in the application of GroEL biosensor biolayer interferometry (BLI) technologies and includes expanded uses of GroEL as a molecular scaffold for electron microscopy determination. The first example presents an extension of the ability to detect dynamic pre-aggregate transients in therapeutic protein solutions where the assessment of the kinetic stability of any folded protein or, as shown herein, quantitative detection of mutant-type protein when mixed with wild-type native counterparts. Secondly, using a BL

SUBMITTER: O'Neil PT 

PROVIDER: S-EPMC5962814 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature