Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis.

ABSTRACT: Purpose:To compare lymphocyte populations present within inflamed eyes in two rat models of autoimmune uveitis. Methods:Experimental autoimmune uveitis (EAU) and primed mycobacterial uveitis (PMU) were initiated in Lewis rats. Aqueous and vitreous were collected at peak inflammation (PMU at day 2, EAU at day 14). The number of cells in the aqueous and vitreous was determined and compared for each eye and between the two models. Intraocular CD-19+ B cells, CD3+ T cells, and CD4+ or CD8+ T-cell subpopulations were identified by flow cytometry and compared between EAU and PMU. Results:The median number of cells/mL collected from PMU aqueous (7.98 × 107 cells/mL), was not significantly different from the number of cells collected from EAU aqueous (1.61 × 107 cells/mL, P = 0.94). EAU aqueous co

SUBMITTER: Pepple KL 

PROVIDER: S-EPMC5963002 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature