Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation.

ABSTRACT:

Background

The SOS response is an almost ubiquitous response of cells to genotoxic stresses. The full complement of genes in the SOS regulon for Vibrio species has only been addressed through bioinformatic analyses predicting LexA binding box consensus and in vitro validation. Here, we perform whole transcriptome sequencing from Vibrio cholerae treated with mitomycin C as an SOS inducer to characterize the SOS regulon and other pathways affected by this treatment.

Results

Comprehensive transcriptional profiling allowed us to define the full landscape of promoters and transcripts active in V. cholerae. We performed extensive transcription start site (TSS) mapping as well as detection/quantification of the coding and non-coding RNA (ncRNA) repertoire in strain N16961. To impro

SUBMITTER: Krin E 

PROVIDER: S-EPMC5963079 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature