Erythrocyte microRNA sequencing reveals differential expression in relapsing-remitting multiple sclerosis.

ABSTRACT:

Background

There is a paucity of knowledge concerning erythrocytes in the aetiology of Multiple Sclerosis (MS) despite their potential to contribute to disease through impaired antioxidant capacity and altered haemorheological features. Several studies have identified an abundance of erythrocyte miRNAs and variable profiles associated with disease states, such as sickle cell disease and malaria. The aim of this study was to compare the erythrocyte miRNA profile of relapsing-remitting MS (RRMS) patients to healthy sex- and age-matched controls.

Methods

Erythrocytes were purified by density-gradient centrifugation and RNA was extracted. Following library preparation, samples were run on a HiSeq4000 Illumina instrument (paired-end 100 bp sequencing). Sequenced erythrocyte miRNA

SUBMITTER: Groen K 

PROVIDER: S-EPMC5963124 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature