Imaging Ca²⁺ with a Fluorescent Rhodol.

ABSTRACT: Ca²⁺ mediates a host of biochemical and biophysical signaling processes in cells. The development of synthetic, Ca²⁺-sensitive fluorophores has played an instrumental role in our understanding of the temporal and spatial dynamics of Ca²⁺. Coupling Ca²⁺-selective ligands to fluorescent reporters has provided a wealth of excellent indicators that span the visible excitation and emission spectrum and possess Ca²⁺ affinities suited to a variety of cellular contexts. One underdeveloped area is the use of hybrid rhodamine/fluorescein fluorophores, or rhodols, in the context of Ca²⁺ sensing. Rhodols are bright and photostable and have good two-photon absorption cross sections (σ_TPA), making them excellent candidates for

SUBMITTER: Contractor AA 

PROVIDER: S-EPMC5963927 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature