An enzyme-linked immunosorbent assay for measuring GPIHBP1 levels in human plasma or serum.

ABSTRACT:

Background

Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1), a glycosylphosphatidylinositol (GPI)-anchored protein of capillary endothelial cells, transports lipoprotein lipase to the capillary lumen and is essential for the lipolytic processing of triglyceride-rich lipoproteins.

Objective

Because some GPI-anchored proteins have been detected in plasma, we tested whether GPIHBP1 is present in human blood and whether GPIHBP1 deficiency or a history of cardiovascular disease affected GPIHBP1 circulating levels.

Methods

We developed 2 monoclonal antibodies against GPIHBP1 and used the antibodies to establish a sandwich enzyme-linked immunosorbent assay (ELISA) to measure GPIHBP1 levels in human blood.

Results

The GPIHBP1 E

SUBMITTER: Miyashita K 

PROVIDER: S-EPMC5963937 | biostudies-literature | 2018 Jan - Feb

REPOSITORIES: biostudies-literature