The COOH-terminal domain of huntingtin interacts with RhoGEF kalirin and modulates cell survival.

ABSTRACT: Human huntingtin (Htt) contains 3144 amino acids and has an expanded polyglutamine region near the NH₂-terminus in patients with Huntington's disease. While numerous binding partners have been identified to NH₂-terminal Htt, fewer proteins are known to interact with C-terminal domains of Htt. Here we report that kalirin, a Rac1 activator, is a binding partner to C-terminal Htt. Kalirin and Htt co-precipitated from mouse brain endosomes and co-localized at puncta in NRK and immortalized striatal cells and primary cortical neurons. We mapped the interaction domains to kalirin674-1272 and Htt2568-3144 and determined that the interaction between kalirin and Htt was independent of HAP1, a known interactor for Htt and kalirin. Kalirin precipitated with mutant Htt was more a

SUBMITTER: McClory H 

PROVIDER: S-EPMC5964228 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature