Microglia remodel synapses by presynaptic trogocytosis and spine head filopodia induction.

ABSTRACT: Microglia are highly motile glial cells that are proposed to mediate synaptic pruning during neuronal circuit formation. Disruption of signaling between microglia and neurons leads to an excess of immature synaptic connections, thought to be the result of impaired phagocytosis of synapses by microglia. However, until now the direct phagocytosis of synapses by microglia has not been reported and fundamental questions remain about the precise synaptic structures and phagocytic mechanisms involved. Here we used light sheet fluorescence microscopy to follow microglia-synapse interactions in developing organotypic hippocampal cultures, complemented by a 3D ultrastructural characterization using correlative light and electron microscopy (CLEM). Our findings define a set of dynamic microglia-syna

SUBMITTER: Weinhard L 

PROVIDER: S-EPMC5964317 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature