A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons.

ABSTRACT: The brain is composed of hundreds of different neuronal subtypes, which largely retain their identity throughout the lifespan of the organism. The mechanisms governing this stability are not fully understood, partly due to the diversity and limited size of clinically relevant neuronal populations, which constitute a technical challenge for analysis. Here, using a strategy that allows for ChIP-seq combined with RNA-seq in small neuronal populations in vivo, we present a comparative analysis of permissive and repressive histone modifications in adult midbrain dopaminergic neurons, raphe nuclei serotonergic neurons, and embryonic neural progenitors. Furthermore, we utilize the map generated by our analysis to show that the transcriptional response of midbrain dopaminergic neurons following 6-

SUBMITTER: Sodersten E 

PROVIDER: S-EPMC5964330 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature