Characterization of Individual Human Antibodies That Bind Pertussis Toxin Stimulated by Acellular Immunization.

ABSTRACT: Despite high vaccination rates, the incidence of whooping cough has steadily been increasing in developing countries for several decades. The current acellular pertussis (aP) vaccines all include the major protective antigen pertussis toxin (PTx) and are safer, but they appear to be less protective than infection or older, whole-cell vaccines. To better understand the attributes of individual antibodies stimulated by aP, we isolated plasmablast clones recognizing PTx after booster immunization of two donors. Five unique antibody sequences recognizing native PTx were recovered and expressed as recombinant human IgG1 antibodies. The antibodies all bind different epitopes on the PTx S1 subunit, B oligomer, or S1-B subunit interface, and just one clone neutralized PTx in an in vitro ass

SUBMITTER: Acquaye-Seedah E 

PROVIDER: S-EPMC5964521 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature