NPBSS: a new PacBio sequencing simulator for generating the continuous long reads with an empirical model.

ABSTRACT:

Background

PacBio sequencing platform offers longer read lengths than the second-generation sequencing technologies. It has revolutionized de novo genome assembly and enabled the automated reconstruction of reference-quality genomes. Due to its extremely wide range of application areas, fast sequencing simulation systems with high fidelity are in great demand to facilitate the development and comparison of subsequent analysis tools. Although there are several available simulators (e.g., PBSIM, SimLoRD and FASTQSim) that target the specific generation of PacBio libraries, the error rate of simulated sequences is not well matched to the quality value of raw PacBio datasets, especially for PacBio's continuous long reads (CLR).

Results

By analyzing the characteristic features of

SUBMITTER: Wei ZG 

PROVIDER: S-EPMC5964698 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature