Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii).

ABSTRACT:

Background

Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process.

Methods

2D-PAGE, NanoLC-MS/MS with higher resolution, Ensembl lamprey and NCBI protein databases, as well as western blot was used to compare the proteomics of buccal gland secretion from China northeast lampreys (Lampetra morii) which had been fed for 0, 10, and 60 min, respectively.

Results

In the present study, the number of identifie

SUBMITTER: Li B 

PROVIDER: S-EPMC5964706 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature