Resveratrol induces autophagy-dependent apoptosis in HL-60 cells.

ABSTRACT:

Background

All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy.

Methods

Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry. Pharmaceutical or genetic inhibition of autophagy involved were carried by 3- methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Differences between two values were tested by Student's unpaired t test.

Results

We show that resveratrol-i

SUBMITTER: Fan Y 

PROVIDER: S-EPMC5964919 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature