The characteristics of vessel lining cells in normal spleens and their role in the pathobiology of myelofibrosis.

ABSTRACT: The CD34^-CD8α⁺, sinusoid lining, littoral cells (LCs), and CD34⁺CD8α^-, splenic vascular endothelial cells (SVECs) represent 2 distinct cellular types that line the vessels within normal spleens and those of patients with myelofibrosis (MF). To further understand the respective roles of LCs and SVECs, each was purified from normal and MF spleens, cultured, and characterized. Gene expression profiling indicated that LCs were a specialized type of SVEC. LCs possessed a distinct gene expression profile associated with cytoskeleton regulation, cellular interactions, endocytosis, and iron transport. LCs also were characterized by strong phagocytic activity, less robust tube-forming capacity and a limited proliferative potential. These characteristics u

SUBMITTER: Qiu J 

PROVIDER: S-EPMC5965045 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature