Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue.

ABSTRACT: We have developed our original tissue engineering technology "cell sheet engineering" utilizing temperature-responsive culture dishes. The cells are confluently grown on a temperature-responsive culture dish and can be harvested as a cell sheet by lowering temperature without enzymatic digestion. Cell sheets are high-cell-density tissues similar to actual living tissues, maintaining their structure and function. Based on this "cell sheet engineering", we are trying to create functional cardiac tissues from human induced pluripotent stem cells, for regenerative therapy and in vitro drug testing. Toward this purpose, it is necessary to evaluate the contractility of engineered cardiac cell sheets. Therefore, in the present study, we developed a contractile force measurement system and evaluat

SUBMITTER: Sasaki D 

PROVIDER: S-EPMC5965888 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature