Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells.

ABSTRACT: : Fractalkine (FKN) is a cytokine which plays an important role in atherosclerosis and other inflammatory diseases. Studies have shown that FKN induces integrin-independent leukocyte adhesion to primary endothelial cells under physiological flow conditions. Further, increased expression of FKN has been demonstrated in atherosclerotic lesions induced by low shear stress. However, the signal transduction mechanisms involved in low shear stress-induced FKN upregulation are not well characterized. In this study, EA.hy926 cells were subjected to varying intensity of fluid shear stress for different time durations. Further, mRNA and protein expressions of FKN were assessed by quantitative real-time PCR and Western blotting, respectively. Upregulation of FKN expression, which was induced via acti

SUBMITTER: Ruze A 

PROVIDER: S-EPMC5965924 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature