Azide-Alkyne Click Conjugation on Quantum Dots by Selective Copper Coordination.

ABSTRACT: Functionalization of nanocrystals is essential for their practical application, but synthesis on nanocrystal surfaces is limited by incompatibilities with certain key reagents. The copper-catalyzed azide-alkyne cycloaddition is among the most useful methods for ligating molecules to surfaces, but has been largely useless for semiconductor quantum dots (QDs) because Cu⁺ ions quickly and irreversibly quench QD fluorescence. To discover nonquenching synthetic conditions for Cu-catalyzed click reactions on QD surfaces, we developed a combinatorial fluorescence assay to screen >2000 reaction conditions to maximize cycloaddition efficiency while minimizing QD quenching. We identify conditions for complete coupling without significant quenching, which are compatible with common QD poly

SUBMITTER: Mann VR 

PROVIDER: S-EPMC5966341 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature