Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation.

ABSTRACT: Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher their molecular composition, to understand their mechanisms, and to find specific elements to target them. However, the specific analysis of invadosomes is challenging, because it is difficult to maintain their integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure the specific identification of invadosome components, we here develop a method that combines laser microdissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this comb

SUBMITTER: Ezzoukhry Z 

PROVIDER: S-EPMC5966458 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature