Deletion of Endo-β-1,4-Xylanase VmXyl1 Impacts the Virulence of Valsa mali in Apple Tree.

ABSTRACT: Valsa mali, a parasitic fungus, is a destructive pathogen of apple tree that causes heavy economic losses in China. The pathogen secretes various cell wall-degrading enzymes (CWDEs) that degrade plant cell-wall components, and thus facilitate its entry into host cells. Therefore, functional analysis of the genes encoding CWDEs is necessary to understand virulence of V. mali toward apple tree. Here, we identified and cloned an endo-β-1,4-xylanase gene, VmXyl1 in V. mali. The full-length cDNA of VmXyl1 is 1626 bp containing 5'- and 3'-non-coding regions, as well an open reading frame of 1320 bp that encodes a protein with a calculated molecular mass and an isoelectric point of 43.8 kDa and 4.4, respectively. The predicted amino acid sequences showed signifi

SUBMITTER: Yu C 

PROVIDER: S-EPMC5966579 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature