Modeling sources of interlaboratory variability in electrophysiological properties of mammalian neurons.

ABSTRACT: Patch-clamp electrophysiology is widely used to characterize neuronal electrical phenotypes. However, there are no standard experimental conditions for in vitro whole cell patch-clamp electrophysiology, complicating direct comparisons between data sets. In this study, we sought to understand how basic experimental conditions differ among laboratories and how these differences might impact measurements of electrophysiological parameters. We curated the compositions of external bath solutions (artificial cerebrospinal fluid), internal pipette solutions, and other methodological details such as animal strain and age from 509 published neurophysiology articles studying rodent neurons. We found that very few articles used the exact same experimental solutions as any other, and some solution dif

SUBMITTER: Tebaykin D 

PROVIDER: S-EPMC5966732 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature