Engineering a nanolab for the determination of lysosomal nitric oxide by the rational design of a pH-activatable fluorescent probe.

ABSTRACT: Nitric oxide (NO) is often involved in many different physiological processes including the regulation of lysosomal functions. However, it remains a great challenge to explore the variations of NO levels in lysosomes, limiting the understanding behind its biological functions in cellular signaling pathways and various diseases. Herein, a pH-activatable fluorescent probe, Rhod-H-NO, was designed and synthesized for the determination of lysosomal NO, in which the activation response model is beneficial towards getting accurate biological information. To ensure that Rhod-H-NO can accumulate effectively and exist stably in lysosomes without interference and degradation from other active species, Rhod-H-NO was engineered into the nanopores of mesoporous silica nanoparticles

SUBMITTER: Li Y 

PROVIDER: S-EPMC5966799 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature