Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.

ABSTRACT:

Background

Transposon-mediated, non-viral gene delivery is a powerful tool for generating stable cell lines and transgenic animals. However, as multi-copy insertion is the preferred integration pattern, there is the potential for uncontrolled changes in endogenous gene expression and detrimental effects in cells or animals. Our group has previously reported on the generation of several transgenic cattle by using microinjection of the Sleeping Beauty (SB) and PiggyBac (PB) transposons and seeks to explore the long-term effects of this technology on cattle.

Results

Transgenic cattle, one female (SNU-SB-1) and one male (SNU-PB-1), reached over 36 months of age with no significant health issues and normal blood parameters. The detection of transgene integration and fluorescent s

SUBMITTER: Yum SY 

PROVIDER: S-EPMC5966871 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature