HMGB1 released from intestinal epithelia damaged by cholera toxin adjuvant contributes to activation of mucosal dendritic cells and induction of intestinal cytotoxic T lymphocytes and IgA.

ABSTRACT: Cholera toxin (CT) is a potent mucosal adjuvant and oral administration of ovalbumin (OVA) antigens plus CT induces OVA-specific CD8⁺ cytotoxic T lymphocytes (CTLs) and IgA production in intestinal mucosa. However, the mechanisms of induction of these immune responses remain unknown. Intestinal OVA-specific CD8⁺ CTLs were not induced by oral administration of the CT active (CTA) or CT binding (CTB) subunit as an adjuvant and CD11c⁺ DCs were involved in cross-priming of intestinal CTLs. CD8⁺CD103⁺CD11c⁺CD11b^-DCs and DCIR2⁺CD103⁺CD11c⁺CD11b⁺ DCs were distributed in the intestinal lamina propria and mesenteric lymph nodes, both DC subsets expressed DEC-205, and the expressi

SUBMITTER: Wakabayashi A 

PROVIDER: S-EPMC5967345 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature