Independent assessment and improvement of wheat genome sequence assemblies using Fosill jumping libraries.

ABSTRACT:

Background

The accurate sequencing and assembly of very large, often polyploid, genomes remains a challenging task, limiting long-range sequence information and phased sequence variation for applications such as plant breeding. The 15-Gb hexaploid bread wheat (Triticum aestivum) genome has been particularly challenging to sequence, and several different approaches have recently generated long-range assemblies. Mapping and understanding the types of assembly errors are important for optimising future sequencing and assembly approaches and for comparative genomics.

Results

Here we use a Fosill 38-kb jumping library to assess medium and longer-range order of different publicly available wheat genome assemblies. Modifications to the Fosill protocol generated longer Illumina sequ

SUBMITTER: Lu FH 

PROVIDER: S-EPMC5967450 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature