Evaluation of tracer labelled methionine load test in vitamin B-12 deficient adolescent women.

ABSTRACT:

Background

Methionine loading test (MLT) has been used primarily to identify defects in transsulfuration of homocysteine in cystathionine beta synthase deficiency. It may not be as useful to evaluate remethylation pathway, in vitamin B-12 and folate deficiencies.

Objective

We used tracer isotope labelled MLT to interrogate transsulfuration and remethylation independently in vitamin B-12 deficiency.

Design

We studied vitamin B-12 deficient women with a tracer labelled MLT before and eleven months after treatment with vitamin B-12. The fractional contribution of [13C]homocysteine to breath CO2 was used as a measure of transsulfuration, and difference in the intracellular enrichment of [13C]methionine and that of [C2H3]methionine as a measure of remethylation of homocyst

SUBMITTER: Bhat DS 

PROVIDER: S-EPMC5967743 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature