Decreased DNA methylation of a CpG site in the HBAP1 gene in plasma DNA from pregnant women.

ABSTRACT:

Objective

The objective of this study is to identify potential CpG site(s) or DNA methylation pattern(s) in the pseudo α-globin 1 gene (HBAP1 gene), the gene which locates in α-thalassemia-1 deletion mutation, to differentiate plasma DNA between pregnant and non-pregnant women.

Method

DNA methylation profiles of placenta and peripheral blood from the MethBase database were compared to screen differentially methylated regions. This region was confirmed the differential by methylation-sensitive high resolution melt (MS-HRM) analysis. The differential region was used to compare DNA methylation profile of plasma DNA between pregnant and non-pregnant women by bisulfite amplicon sequencing in three levels: overall, individual CpG sites and individual molecules (DNA methylation pat

SUBMITTER: Pangeson T 

PROVIDER: S-EPMC5967787 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature