Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.

ABSTRACT:

Background

Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for human trichinellosis, but the major drawback of the ES antigens for assaying anti-Trichinella antibodies is the false negative in the early Trichinella infection period. The aim of this study was to characterize the T. spiralis putative serine protease (TsSP) and to investigate its potential use for diagnosis of trichinellosis.

Methodology/principal findings

The full-length TsSP sequence was cloned and expressed, and recombinant TsSP (rTsSP) was purified by Ni-NTA-Sefinose C

SUBMITTER: Sun GG 

PROVIDER: S-EPMC5967804 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature