Ontology highlight
ABSTRACT:
SUBMITTER: Bao X
PROVIDER: S-EPMC5967874 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Nature methods 20180212 3
We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed ...[more]