In Vivo Superresolution Imaging of Neuronal Structure in the Mouse Brain.

ABSTRACT:

Objective

this study proposes and evaluates a technique for in vivo deep-tissue superresolution imaging in the light-scattering mouse brain at up to a 3.5 Hz 2-D imaging rate with a 21×21 μm² field of view.

Methods

we combine the deep-tissue penetration and high imaging speed of resonant laser scanning two-photon (2P) microscopy with the superresolution ability of patterned excitation microscopy. Using high-frequency intensity modulation of the scanned two-photon excitation beam, we generate patterned illumination at the imaging plane. Using the principles of structured illumination, the high-frequency components in the collected images are then used to reconstruct images with an approximate twofold increase in optical resolution.

Results

using our techniqu

SUBMITTER: Urban BE 

PROVIDER: S-EPMC5967880 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature