Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt.

ABSTRACT: Two deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques and several isolates were recovered in pure culture from various depths in both boreholes. Although no archaeal isolates were obtained in pure culture, strict anaerobes and facultative anaerobic bacteria belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were isolated and identified using the 16S rRNA gene sequence. Analysis of three selected enrichment cultures by amplification of both bacteri

SUBMITTER: Leandro T 

PROVIDER: S-EPMC5968172 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature