Putative bovine topological association domains and CTCF binding motifs can reduce the search space for causative regulatory variants of complex traits.

ABSTRACT:

Background

Topological association domains (TADs) are chromosomal domains characterised by frequent internal DNA-DNA interactions. The transcription factor CTCF binds to conserved DNA sequence patterns called CTCF binding motifs to either prohibit or facilitate chromosomal interactions. TADs and CTCF binding motifs control gene expression, but they are not yet well defined in the bovine genome. In this paper, we sought to improve the annotation of bovine TADs and CTCF binding motifs, and assess whether the new annotation can reduce the search space for cis-regulatory variants.

Results

We used genomic synteny to map TADs and CTCF binding motifs from humans, mice, dogs and macaques to the bovine genome. We found that our mapped TADs exhibited the same hallmark properties of th

SUBMITTER: Wang M 

PROVIDER: S-EPMC5968476 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature