Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging.

ABSTRACT:

Background

Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level.

Results

We report the use of multimodal Raman and mass spectrometry imaging to study the cellular-level biochemical changes that occur from silencing the phytoene desaturase (pds) gene using a Foxtail mosaic virus (FoMV) vector in maize leaves. The multimodal imaging method allows the localized carotenoid distribution to be measured and reveals differences lost in the spatial average when analyzing a carotenoid extraction of the whole leaf. The nature of the Raman and mass spectrometry signals are complementar

SUBMITTER: Burkhow SJ 

PROVIDER: S-EPMC5968576 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature