Cannabinoid CB2 receptors in the mouse brain: relevance for Alzheimer's disease.

ABSTRACT: BACKGROUND:Because of their low levels of expression and the inadequacy of current research tools, CB2 cannabinoid receptors (CB2R) have been difficult to study, particularly in the brain. This receptor is especially relevant in the context of neuroinflammation, so novel tools are needed to unveil its pathophysiological role(s). METHODS:We have generated a transgenic mouse model in which the expression of enhanced green fluorescent protein (EGFP) is under the control of the cnr2 gene promoter through the insertion of an Internal Ribosomal Entry Site followed by the EGFP coding region immediately 3' of the cnr2 gene and crossed these mice with mice expressing five familial Alzheimer's disease (AD) mutations (5xFAD). RESULTS:Expression of EGFP in control mice was below the level of detection

SUBMITTER: Lopez A 

PROVIDER: S-EPMC5968596 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature