Ontology highlight
ABSTRACT:
SUBMITTER: Liu W
PROVIDER: S-EPMC5968841 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
International journal of biological sciences 20180405 5
Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to systemically examine cellular activities and the impact of medium components in high-density culture. We show that medium acidosis is the main factor that alters cell cycle, gene exp ...[more]