Fluorogenic Ag⁺ -Tetrazolate Aggregation Enables Efficient Fluorescent Biological Silver Staining.

ABSTRACT: Silver staining, which exploits the special bioaffinity and the chromogenic reduction of silver ions, is an indispensable visualization method in biology. It is a most popular method for in-gel protein detection. However, it is limited by run-to-run variability, background staining, inability for protein quantification, and limited compatibility with mass spectroscopic (MS) analysis; limitations that are largely attributed to the tricky chromogenic visualization. Herein, we reported a novel water-soluble fluorogenic Ag⁺ probe, the sensing mechanism of which is based on an aggregation-induced emission (AIE) process driven by tetrazolate-Ag⁺ interactions. The fluorogenic sensing can substitute the chromogenic reaction, leading to a new fluorescence silver staining metho

SUBMITTER: Xie S 

PROVIDER: S-EPMC5969303 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature