Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements.

ABSTRACT: RNA polymerase (Pol) III transcribes small untranslated RNAs that are essential for cellular homeostasis and growth. Its activity is regulated by inactivation of tumor suppressor proteins and overexpression of the oncogene c-MYC, but the concerted action of these tumor-promoting factors on Pol III transcription has not yet been assessed. In order to comprehensively analyse the regulation of Pol III transcription during tumorigenesis we employ a model system that relies on the expression of five genetic elements to achieve cellular transformation. Expression of these elements in six distinct transformation intermediate cell lines leads to the inactivation of TP53, RB1, and protein phosphatase 2A, as well as the activation of RAS and the protection of telomeres by TERT, thereby conducting to

SUBMITTER: Durrieu-Gaillard S 

PROVIDER: S-EPMC5969553 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature