Genetic Deletion of IL-19 (Interleukin-19) Exacerbates Atherogenesis in Il19^-/-×Ldlr^-/- Double Knockout Mice by Dysregulation of mRNA Stability Protein HuR (Human Antigen R).

ABSTRACT:

Objective

To test the hypothesis that loss of IL-19 (interleukin-19) exacerbates atherosclerosis. APPROACH AND RESULTS: Il19^-/- mice were crossed into Ldlr^-/- (low-density lipoprotein receptor knock out) mice. Double knockout (dKO) mice had increased plaque burden in aortic arch and root compared with Ldlr^-/- controls after 14 weeks of high-fat diet (HFD). dKO mice injected with 10 ng/g per day rmIL-19 had significantly less plaque compared with controls. qRT-PCR and Western blot analysis revealed dKO mice had increased systemic and intraplaque polarization of T cells and macrophages to proinflammatory T_h1 and M1 phenotypes, and also significantly increased TNF (tumor necrosis factor)-α expression in spleen and aortic

SUBMITTER: Ray M 

PROVIDER: S-EPMC5970062 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature