A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide.

ABSTRACT: Hydrogen sulfide (H₂S) is an important gasotransmitter. Although a large number of fluorescent probes for cellular H₂S have been reported, only a few can detect H₂S in mitochondria, a cellular organelle connecting H₂S with mitochondrial function and metabolic pathways. We hereby describe a novel near-infrared fluorescent probe, nimazide, by introducing sulfonyl azide to the core structure of a QSY-21 dark quencher. Nimazide responded quickly to H₂S, resulting in robust fluorescence turn-off changes. This conversion displayed high specificity and fast kinetics. More impressively, we observed a robust fluorescence decrease in live cells loaded with mitochondrial nimazide in response to extracellular addition of nanomolar H₂S,

SUBMITTER: Ji A 

PROVIDER: S-EPMC5970079 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature